說明
資料紀錄
此資源出現紀錄的資料已發佈為達爾文核心集檔案（DwC-A），其以一或多組資料表構成分享生物多樣性資料的標準格式。 核心資料表包含 205 筆紀錄。
此 IPT 存放資料以提供資料儲存庫服務。資料與資源的詮釋資料可由「下載」單元下載。「版本」表格列出此資源的其它公開版本，以便利追蹤其隨時間的變更。
版本
以下的表格只顯示可公開存取資源的已發布版本。
如何引用
研究者應依照以下指示引用此資源。:
Pellegrino M, Sánchez J, Poljak S (2026). Mammal diversity in remote and harsh habitats: validating the power of environmental DNA metabarcoding at the southernmost tip of South America. Version 1.0. CADIC. Occurrence dataset. https://ipt.mincyt.gob.ar/resource?r=cadic-metabarcoding&v=1.0
權利
研究者應尊重以下權利聲明。:
此資料的發布者及權利單位為 Centro Austral de Investigaciones Científicas - CADIC CONICET。 This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.
GBIF 註冊
此資源已向GBIF註冊，並指定以下之GBIF UUID: 72576e57-9a9d-4c05-9c5b-8b035ee44a49。 Centro Austral de Investigaciones Científicas - CADIC CONICET 發佈此資源，並經由GBIF Argentina同意向GBIF註冊成為資料發佈者。
關鍵字
Occurrence; Observation; eDNA; metabarcoding; Tierra del Fuego; Argentina; freshwater; biodiversity
聯絡資訊
- 元數據提供者 ●
- 出處 ●
- 連絡人
https://orcid.org/0009-0007-5002-4560
- 出處
- 出處
地理涵蓋範圍
The dataset includes freshwater sampling sites distributed across Tierra del Fuego Province, southern Argentina. Samples were collected from five river systems representing a range of environments: Río Olivia, Río Lasifashaj, Río Milna, Río Ewan Norte, and Río Grande.
| 界定座標範圍 | 緯度南界 經度西界 [-54.737, -68.599], 緯度北界 經度東界 [-53.199, -66.643] |
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分類群涵蓋範圍
All mammal taxa detected in freshwater samples were identified to species level using environmental DNA metabarcoding.
| Species | Ondatra zibethicus (Muskrat), Euneomys chinchilloides (Patagonian chinchilla mouse), Castor canadensis (American beaver), Lycalopex griseus (Gray fox), Lycalopex culpaeus (Red fox), Lama guanicoe (Guanaco), Bos taurus (Domestic cattle), Oligoryzomys longicaudatus (Long-tailed Colilargo), Abrothrix xanthorhina (Yellow-nosed Grass Mouse), Lontra provocax (Southern river otter), Canis lupus domesticus (dog), Rattus rattus (Black rat), Ctenomys magellanicus (Ctenomys magellanicus), Neogale vison (American mink), Abrothrix longipilis (Long-haired grass mouse), Sus scrofa (Wild boa), Rattus norvegicus (Norway rat) |
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Additionally, two fish species were detected in the samples and identified to species level.
| Species | Salmo trutta (Brown trout), Oncorhynchus mykiss (Rainbow trout) |
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時間涵蓋範圍
| 起始日期 | 2024-04-16 |
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計畫資料
This project evaluated the effectiveness of environmental DNA (eDNA) metabarcoding for detecting mammal species in freshwater ecosystems of Tierra del Fuego, Argentina. Water samples collected from five river systems were analyzed using MiMammal-U primers targeting the mitochondrial 12S rRNA gene and high-throughput sequencing. The project aimed to validate eDNA metabarcoding as a biodiversity monitoring tool in remote and environmentally challenging regions and to optimize protocols for its future application in wildlife monitoring and conservation programs in Tierra del Fuego.
| 計畫名稱 | Mammal diversity in remote and harsh habitats: validating the power of environmental DNA metabarcoding at the southernmost tip of South America |
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| 經費來源 | This work was funded the Consejo Nacional de Investigaciones Científicas y Técnicas (PIP 11220210100691CO 2022–2024) and Fundación Williams (Project 630). |
| 研究區域描述 | The study was conducted in Tierra del Fuego Province, southern Argentina. Freshwater samples were collected from five river systems (Grande, Ewan, Milna, Lasifashaj, and Olivia Rivers) representing a range of environments including steppe, ecotone, forest, and mountain ecosystems. |
| 研究設計描述 | The project evaluated the use of environmental DNA (eDNA) metabarcoding for detecting mammal species in freshwater ecosystems of Tierra del Fuego. At each river, four replicate water samples were collected and filtered for eDNA analysis. DNA was extracted from filters, amplified targeting a fragment of the mitochondrial 12S rRNA gene using MiMammal-U primers, and sequenced on an Illumina MiSeq platform. Taxonomic assignments were performed using a curated local reference database and a standardized bioinformatic workflow. The resulting occurrence records were used to assess mammalian diversity and evaluate the effectiveness of eDNA metabarcoding as a biodiversity monitoring tool. |
參與計畫的人員:
取樣方法
Water samples for this eDNA study were collected from five river sites in TdF Province on April 16, 2024. The sampling sites included the Grande, Ewan, Milna, Lasifashaj, and Olivia Rivers. At each site, four 5 L replicate water samples were collected at the same sampling location (n = 20 L per site) to maximize the detection of rare species. Sampling was conducted using sterile gloves to prevent cross-contamination. Each replicate was obtained by submerging a sterile container submerged just below the surface, upstream from the operator, to minimize the risk of contamination. Samples were transported and stored in a cold chamber at 4 °C. Water filtration was performed within 72 hours using sterile microbiological monitors with 0.45 µm pore diameter nitrocellulose filters to retain cellular material. Although the initial sample volume was 5 L, effective filtration was limited to 1 L per replicate due to high sediment loads. To monitor for potential contamination during sampling and filtration, a 5 L field blank of distilled water was treated under the identical conditions. Filters were subsequently stored at -80 °C until DNA extraction.
| 研究範圍 | Temporal coverage: 16-Apr-2024 to 16-Apr-2024 Frequency of sampling: One-time sampling event Sampling effort: 20 samples, 5 rivers, 4 replicates per site Study extent: Tierra del Fuego, Argentina |
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| 品質控管 | Field and laboratory controls were included to minimize contamination and ensure data quality. A field blank consisting of distilled water was processed alongside environmental samples during sampling and filtration. Negative PCR controls were included during library preparation. Sequence quality was assessed using FastQC, and raw reads were filtered, trimmed, merged, and screened for chimeras prior to taxonomic assignment. Taxonomic identification was performed using a curated reference database. Species occurrences were retained only after applying bioinformatic. |
方法步驟描述:
- 1. Collection of freshwater samples from five river systems in Tierra del Fuego. 2. Filtration of water samples through 0.45 µm nitrocellulose membranes. 3. Extraction of environmental DNA using a CTAB-based protocol. 4. Amplification of the mitochondrial 12S rRNA gene using MiMammal-U primers. 5. High-throughput sequencing on an Illumina MiSeq platform (2 × 300 bp). 6. Bioinformatic processing including quality filtering, read merging, chimera removal, and OTU generation. 7. Taxonomic assignment using a curated local reference database. 8. Generation of occurrence records from validated species detections.
額外的詮釋資料
| Introduction | |
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| Getting Started | |
| 目的 | |
| 維護說明 | This dataset is associated with a specific research project. |